Comparative proteomics of Rhizopus delemar ATCC 20344 unravels the role of amino acid catabolism in fumarate accumulation
نویسندگان
چکیده
The filamentous fungus Rhizopus delemar naturally accumulates relatively high amounts of fumarate. Although the culture conditions that increase fumarate yields are well established, the network underlying the accumulation of fumarate is not yet fully understood. We set out to increase the knowledge about fumarate accumulation in R. delemar. To this end, we combined a transcriptomics and proteomics approach to identify key metabolic pathways involved in fumarate production in R. delemar, and propose that a substantial part of the fumarate accumulated in R. delemar during nitrogen starvation results from the urea cycle due to amino acid catabolism.
منابع مشابه
Copper accumulation and phosphatase activities of Aspergillus and Rhizopus.
Copper accumulation and phosphatase activities of three Aspergillus species resistant to copper were compared to three copper-sensitive Rhizopus species. High level of acid phosphatases and decreased Cu2+-uptake were found with resistant in contrast to sensitive strains. The presence of copper(II) ions in the medium increased the production of acid phosphatases in the resistant A. niger and dec...
متن کاملSpecies and Varieties in the Rhizopus Arrhizus-rhizopus Oryzae Group as Indicated by Their Dna Complementarity
DNA renaturation experiments gave evidence to conclude that Rhizopus arrhizus, R. OIyzae, R. delemar, Amylomyces rou.xii, R. delemar var. minimus, R. delemar var. multiplicisporus, R. arrhizus var. delemar, R. chungkuoensis var. isqfermentarius, and R. javanicus var. kawasakiensis can be accommodated in three taxa, namely, R. arrhizus var. arrhizus, R. arrhizus var. rozedi, and R. arrhizus var....
متن کاملRemoval of heavy metals from aqueous solution using Rhizopus delemar mycelia in free and polyurethane-bound form.
This study assesses the ability of mycelia of Rhizopus delemar (both free and immobilized on polyurethane foam) to remove heavy metals from single-ion solutions as well as from a mixture of them. All experiments were conducted using 0.5-5 mm solutions of CuSO4 x 5H2O, CoCl2-6H2O and FeSO4 7H2O. Mycelia immobilized on polyurethane foam cells showed some times increase in uptake compared with tha...
متن کاملMechanism of fumaric acid accumulation in Rhizopus nigricans.
It is doubtful that the glyoxylate bypass plays a significant role in the accumulation of fumaric acid by fungi, as has been postulated. In high glucose media, which favor fumarate production, isocitrate lyase (threo-D(s) isocitrate glyoxylate lyase), which is the key enzyme of the glyoxylate bypass, is strongly repressed. The specific activity of this enzyme remains low as long as glucose is p...
متن کاملSustainable carbon sources for microbial organic acid production with filamentous fungi
BACKGROUND The organic acid producer Aspergillus oryzae and Rhizopus delemar are able to convert several alternative carbon sources to malic and fumaric acid. Thus, carbohydrate hydrolysates from lignocellulose separation are likely suitable as substrate for organic acid production with these fungi. RESULTS Before lignocellulose hydrolysate fractions were tested as substrates, experiments wit...
متن کامل